Author + information
- Tobias Koppara1,
- Kristina Euller2,
- Kazuyuki Yahagi1,
- Hiroyoshi Mori1,
- Oscar David Sanchez1,
- Frank Kolodgie1,
- Eric Wittchow1,
- Michael Joner1 and
- Renu Virmani1
Neoatherosclerosis has been recognized as important disease entity found in nascent neointimal tissue in the aftermath of stent implantation. This study aimed to establish a novel animal model of neoatherosclerosis and to investigate the feasibility in detecting neoatherosclerosis using optical coherence tomography (OCT).
Stented arteries in an atherosclerotic rabbit model (n=15) as well as 6 autopsy cases with neoatherosclerosis were evaluated. The animal model employed 3 different study groups which differed in the duration of cholesterol diet and in the sequence of vascular interventions. OCT frames and histological cross sections were co-registered and neoatherosclerotic changes were assessed by peak intensity and attenuation index of OCT light emission.
The presence of neoatherosclerosis was significantly increased in rabbits with long-term high dose - cholesterol diet and repeat denudation of the endothelial layer.
The cut-off in peak intensity for detecting neoatherosclerosis in human autopsy cases was 117.98 (Specificity: 59.80%; Sensitivity: 75%) and 95.99 in rabbits (Specificity: 62.11%; Sensitivity: 72.90%). The attenuation index was -0.7345 (Specificity: 84.31%; Sensitivity: 77.38%) in humans and 0.2414 in rabbits (Specificity: 89.83%; Sensitivity: 45.16%).
Neoatherosclerotic lesions can be established in a preclinical rabbit model following hypercholesterolemic feeding and repeat arterial denudation. The detection of neoatherosclerosis is feasible using OCT imaging. However, limitations remain in the differentiation of advanced stages of neoatherosclerosis.