|Biomarker||Kidney norepinephrine levels (high-throughput tandem mass spectrometry)||Evaluation of the extent of renal sympathetic denervation|
|Macroscopic evaluation||TTC staining of renal artery||To assess the extent of arterial wall injury|
|Standard light microscopy and morphometry||Semiquantitative scoring of pathological changes (grade 0 to grade 4): nerve, renal artery, vein, arterioles, fat, and collagen||To determine the extent of nerve injury and arterial and surrounding soft-tissue injury|
|Quadrant analysis (0 to 4 quadrants) for nerve injury and soft-tissue injury||Evaluation of circumferential extent of injury|
|Depth of tissue injury, mm||Evaluation of therapeutic penetration|
|Immunohistochemistry||Semiquantitative scoring of intensity of immunostaining (score 0 to score 3) by anti–tyrosine hydroxylase||Evaluation of the ability of the nerves to generate norepinephrine (functional nerve injury)|
TTC = 2,3,5-triphenyltetrazolium chloride.