Reparative Effects of Allogeneic Mesenchymal Precursor Cells Delivered Transendocardially in Experimental Nonischemic Cardiomyopathy
Peter J. Psaltis, Angelo Carbone, Adam J. Nelson, Dennis H. Lau, Troy Jantzen, Jim Manavis, Kerry Williams, Silviu Itescu, Prashanthan Sanders, Stan Gronthos, Andrew C.W. Zannettino and Stephen G. Worthley
Necropsy slices of left ventricle display regions of midmural (white arrowhead) and subepicardial (white arrow) pallor from the placebo (A) and mesenchymal precursor cells (MPC) (B) groups. Masson's trichrome sections show foci of replacement fibrosis (black arrow) in a placebo-treated heart (C) and perivascular fibrosis (black arrowhead) after cell therapy (D). Both treatment groups had increased fibrosis burden compared with healthy control hearts, although this was less in the MPC cohort (1-way analysis of variance p < 0.001) (E). There were trends toward reduced fibrosis in all myocardial walls of MPC sheep (2-way analysis of variance p < 0.01), reaching statistical significance in the septum (F). Sheep that had received cell transplantation had a higher proportion of myocardial segments with negligible fibrosis and fewer segments with either reactive or replacement fibrosis (G). *p < 0.05. Abbreviations as in Figures 1 and 2.