Proliferating Cellular Phenotype in Cell-Treated and Saline Control Cells
Double immunohistochemical detection of proliferating 5-bromo-2'-deoxyuridine-positive cells with von Willebrand factor-positive endothelial cells (A to D) and cardiac myocytes (E to H) within the border zone of rat infarcted myocardium was determined 2 weeks after transplantation of saline control cells (A and E), B cells (B and F), cultured B cells (C and G), and B cells cocultured with nonhematopoietic stem cells (D and H). Positive 5-bromo-2'-deoxyuridine-labeled cells were visualized using 3, 3'-diaminobenzidine in which all positive cells are stained brown and von Willebrand factor and sarcomeric actin staining was visualized using Vector red. Double positive staining is denoted by white arrows. All nuclei were stained with hematoxylin. Instrument magnification 400×. Scale bars are 50 μm.