Purity and Characterization of Selected B Cells From Rat Bone Marrow
Flow cytometric analysis of bone marrow-derived CD45RA-positive B cells revealed that the injected population of CD45RA selected B cells were relatively free of other lineage-positive bone marrow cells including cytotoxic T cells (CD8+) (B), natural killer T cells (CD3) (C), helper T cells (CD4) (D), and neutrophils (E). Isotype control is shown in A. Flow cytometric analysis of bone marrow-derived CD45RA-positive B cells confirmed the majority of the B cells are CD45R-positive lymphocytes (G). IgM-positive immature B cells (H), IgG-positive mature B cells (I), and a minority of early c-kit+ positive pro-B cells (L). The bone marrow-derived B cell fraction did not contain CD5-positive marginal cells (J). Isotype control for CD45R, IgM, IgG, and CD5 antibodies is shown in F, and isotype control for c-kit is shown in K. Representative flow cytometry data are shown.