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Monocytes are crucially involved in all stages of atherogenesis as cellular drivers of vascular inflammation hallmarking atherosclerotic disease.CD16+monocytes are pro-inflammatory cells, whose proportion is related to the occurrence of coronary artery disease (CAD), intma-media thickness and plaque stability. Interleukin-6 (IL-6) and highly sensitive C reactive protein (hs-CRP) were also closely related to atherosclerotic disease.
We investigated the relationship between the monocyte subsets, IL-6,and hs-CRP with the severity of CAD assessed by coronary angiography (CAG) in patients with stable angina pectoris (SAP)through their correlation with Gensini score.
Our study included 45 SAP patients who underwent diagnostic CAG. Thirty two patients of them who diagnosed as CADwere subdivided into 2 groups: 17 patients with multiple-vessel disease (MVD) and 15 patients with single-vessel disease (SVD). The rest thirteen SAPpatients without CAD (non-CAD) were considered as a comparative group. Gensini score was used to assess the severity of CAD. Monocyte subsets were analyzed by flow cytometry and serum levels of IL-6 and hs-CRP were measured by ELISA.
The relative proportion of CD14+ CD16+ and CD14brightCD16+ was significantly higher in CAD patients, MVD and SVD as compared with non-CAD patients and in MVD more than SVD. Serum levels of IL-6 and hs-CRP were significantly increased in CAD patients, MVD and SVD when compared with non-CAD patients, but no significant difference between MVD and SVD. The proportion of CD14+CD16+and CD14brightCD16+ monocytes was positively correlated with Gensini score (r=0.667, P =0.000, r=0.695, P=0.000).
Elevated proportion of CD14+ CD16+ monocytes subsets was associated with the severity of CAD in patients with SAP.