Author + information
- Marjeta Zorc1,
- Ruda Zorc Pleskovic1,
- Ales Pleskovic2,
- Olga Vraspir Porenta1,
- Danijel Petrovic1,
- Dusan Suput1 and
- Aleksandra Milutinovic Zivin1
Coronary artery disease (CAD) is characterized by atherosclerotic changes in vessel wall. Normal histophysiology of tunica media is prerequisite for optimal vessel function. Tunica media is surrounded by adventitial tissue and by perivascular fat tissue (FT) with endocrine properties.
The goal of the research was to define the influence of inflammation in FT on morphology of tunica media.
Endarterectomy was performed in coronary arteries (CAs) from 100 symptomatic patients with diffuse obstructive CAD. In the study, 22 CAs that contained FT in adventitia were enrolled. Analysis was performed in vessel sections, stained with haematoxylin-eosin, Movat, and immunohistochemically for leukocyte common antigen. These CAs was divided into 2 groups: FT was not infiltrated (group-0, N=13) and infiltrated (group-1, N=9). Out of 22 observed CAs in tunica media, the outer elastic membrane was estimated as absent (0) and present (1). In tunica media the intensity of inflammatory cells infiltration was estimated as absent (0), minor (1), and major (2). Study was approved by the National Medical Ethics Committee (MEC 170/07/13, MEC 110/03/16).
Mann Withney test showed that the elastic membrane was more frequently preserved in group-1 than in group-0 (p=0.004) and that the intensity of inflammatory infiltration was more prominent in group-0 than in group-1 (p=0.014). We found significant positive correlation between CAs with presence of inflammatory cells infiltration in FT and presence of outer elastic membrane (p=0.002, Spearman’s correlation). We found significant negative correlation between CAs with presence of inflammatory cells infiltration in FT and intensity of inflammatory cell infiltration around intramural vasa vasorum in tunica media (p=0.010).
The presence of inflammatory infiltrated endocrine FT protects tunica media from the disintegration of the elastic membrane and inflammation.