Author + information
- Jung-Joon Cha1,
- Yangkyu Park2,
- Joho Yun1,
- Juhun Lim1,
- Sang-Jin Lee2,
- Jinhwan Kim2,
- Kwanghyun Kim2 and
- Jong-Hyun Lee3
- 1Department of Biomedical Science and Engineering, Gwangju Institute of Science and Technology(GIST), Gwangju, Republic of Korea
- 2School of Mechanical Engineering, Gwangju Institute of Science and Technology(GIST), Gwangju, Republic of Korea
- 3Department of Biomedical Science and Engineering, Gwangju Institute of Science and Technology(GIST), School of Mechanical Engineering, Gwangju Institute of Science and Technology(GIST), Gwangju, Republic of Korea
In cardiovascular senescence research, various biomarkers such as senescence associated β-galactosidase activity and telomerase activity have been used to analyze the aging process. Nonetheless, biomarkers have several limitations that require complicated procedures but only generate comparative data. Recently, cell aging has been quantitatively analyzed using cell electrical impedance by micro-electrochemical impedance spectroscopy for diagnosis of senescence (MEDoS).
Three groups (3, 4, and 18-month-old) of isogenic zebrafish were prepared for both vascular endothelial cells (fli1a:EGFR) and cardiomyocytes (cmlc2:EGFR). To obtain single cell of each cell type, florescence activating cell sorting system was used. Then, the sorted cells were infused into the MEDoS to measure their cell impedance (n=30 for each cell type in each age group), which were presented in terms of resistance and reactance.
The opposite tendencies of cell impedance could be seen in two cell types (Figure 1). In vascular endothelial cells during aging, resistance monotonously increased, whereas reactance decreased and increased sequentially. In contrast, in cardiomyocytes, resistance continuously decreased, whereas reactance increased and decreased sequentially.
The cell impedance can overcome limitations in previous studies by obtaining quantitative cellular information in real-time without using biomarkers. We believe that changes in cell impedance during aging are affected by changes in the cellular compartment including reactive oxygen species increments and autophagy activity disorder. In this context, cell impedance will be an important analytical factor in the study of aging providing accurate data for understanding senescence as well as cardiovascular diseases.